This pcr system can operate in three modes, stand alone, minsatellite, or as a daisy chained pc controlled network. Eppendorf mastercycler pro pcr system proprietary tect technology reduces evaporation to a minimum, improving reproducibility and specificity. Hardshell pcr plates represent an innovative concept in pcr plate design. This mastercycler ep realplex software module makes it possible to observe the generated raw data in real time, which means that you can immediately evaluate your realtime pcr and interrupt the reaction as soon as the desired result is obtained. This item has been discontinued by the manufacturer and is no longer available. This pcr machine features steadyslope and tect technology, to keep temperature rates consistent, and reduce evaporation and allowing users. Eppendorf 950030010 view more versions of this product. The results obtained through the mastercycler ep realplex are consistent, reproducible and accurate. Five different colors can help organize your workflow. Mastercycler x50 cycler, pcr eppendorf deutschland. A minimal number of moving parts in the optical detection module minimises the vulnerability of the overall system and promotes consistently high sensitivity in. Combine any mix of mastercycler pro gradient s, mastercycler pro gradient, and mastercycler pro 384 to a network.
Ephb3 signaling induces cortical endothelial cell death. Mastercycler ep realplex from eppendorf biocompare. Configuration of the database tool is only accessible for an administrator. Mastercycler x50 cyclers, pcr eppendorf international. To check for the amplification specificity, melting temperature analysis was performed and the expected size of the pcr products was checked on 1.
It should be ascertained that the pcr plates are correctly situated in the block. Tips and tricks helpful infographics to support your daily work. Imazalil exposure induces gut microbiota dysbiosis and. Smaller assays fit nicely on the 32wellblock larger assays can exceed 48 samples and run on the 64wellblock. Mastercycler ep realplex realtime pcr solutions eppendorf has developed a realtime pcr system that meets the requirements of the latest quantitative pcr applications. The mastercycler x50 family boasts ramp rates of up to 10 cs and is the ideal cycler family for routine applications as well as advanced research in molecular biology. Also used to facilitate other temperaturesensitive reactions, the devices can complete restriction enzyme digestion or rapid diagnostics.
Realtime pcr detection and quantification of selected. Eppendorf 950017007 cycle manager prosoftware for mastercycler pro thermal cyclers. Softwarefirmware revisions are made available at no charge through this web page so that all users can benefit from the latest. In our laboratory, we use it routinely for quantitative mrna expression studies, verification of microarray results and pathogen mycoplasma detection in eukaryotic systems. Each cycler with a touchscreen can be networked with nine additional eco units. We operate in more than 30 countries and deliver an extensive portfolio of mission critical products, services and solutions. Mastercycler mastercycler gradient operating manual. Eppendorf mastercycler pro pcr system mastercycler pro. The mastercycler x50 family consist of units with a userfriendly visionize touchscreen interface or eco units which are externally controlled. Eppendorf is a leading life science company that develops and sells instruments, consumables and services for laboratories worldwide. Our mastercycler pro has a special lid to avoid evaporation and superior. Mastercycler ep realplex software setup for the quantifast.
The skirt and deck are molded from a rigid thermostable polymer. Learn more about thermal cyclers, mastercycler ep realplex. Data were analyzed with the use of graphpad prism 6 software graphpad software and mastercycler ep realplex software version 2. Eppendorf mastercycler gradient pcr thermal cycler model 5331. Plant varieties expressing the bt bacillus thuringiensis insecticidal proteins cry1ah and cry2ab have potential commercialization prospects in china. Eppendorf mastercycler ep realplex forums questions. The cotton aphid aphis gossypii is a worldwide pest that damages various important crops.
Modified slip cover light transmissive cover for optical openings in heated platen assembly, us patent pending. The mastercycler nexus pcr cycler provides scientists with flexibility in many forms. Eppendorf north america laboratory equipment, supplies. Connection of several mastercycler ep to one control. No idea why most pcr machine manufacturers make integrated systems with. The mastercycler epgradient s pcr from eppendorf offers a flexibility, gradient heating with responsive heating and cooling. Eppendorf training become an expert with our training program. The most important aspects of current real time quantitative pcr strategies, instrumentation and software and the application of qpcr technology in various areas of applied microbiology. Eppendorf mastercycler ep realplex realtime pcr system. Our mastercycler pro has a special lid to avoid evaporation and superior block homogeneity to achieve consistent results, despite the well being used. Its advanced, highspeed gradient thermal block design makes the ramp heat up fairly fast and allows for a temperature gradient option.
Connection of mastercycler ep realplex to a computer. The eppendorf mastercycler and mastercycler gradient are pcr devices for all molecular biological and biochemical laboratories in the. Expression levels of target genes were normalized to. According to these regulations, any devices supplied after. Software 0 system 0 video conferencing system 0 electrophoresis 45 accessory 1. Eppendorf limited product warranty mastercycler ep. The most advanced configuration of a 96well silver block with the realplex optical module offers highspeed heating and cooling rates and the capacity to. Smaller assays fit nicely on the 32wellblock larger assays can exceed 48 samples. The modular design of eppendorfs mastercycler ep realplex offers researchers a choice of two thermomodules and two optical detection units, depending on their application requirements. Mesenchymal stromal cell delivery of fulllength tumor. The rearing companies that provided the samples analysed in this study claimed that no.
Mastercycler ep realplex fluidigm biomark roche lightcycler 1. Both realplex machines use the same software interface, and its a great one, which is a piece of cake to use. This allows two pcr users in the lab to start and end their pcr reactions whenever they want. The eppendorf mastercycler gradient pcr thermal cycler model 5331 is designed for molecular biological and biochemical research laboratories. Eppendorf mastercycler nexus x2 thermal cyclers e6336000023 print share eppendorf mastercycler nexus x2 thermal cyclers. It is important that they meet your needs and expectations. Because of its high speed, we perform as many as 5 runs a day in our lab, with reliable and accurate results. Used eppendorf mastercycler ep realplex 2 realtime pcr. Mastercycler ep realplex from eppendorf selectscience.
Unfortunately, instrument vendors have not consistently conformed to any. The mastercycler nexus x2 gives you the ability to run two totally independent protocols at the same time. Samples were run on an eppendorf mastercycler ep realplex quiagen and analyzed using realplex software version 2. The mastercycler ep realplex features the latest technology, united with good design and a quality finish. Mastercycler service get your service for absolute confidence. However, their potential effects on nontarget arthropods ntas remain uncharacterized. Two totally independent protocols can be run on the two blocks. Avantor is a trusted global partner to customers and suppliers in the life science, advanced technology and applied materials industries. The 96 well aluminium block can accomodate several standard vessels types from 0. Imazalil exposure induces gut microbiota dysbiosis and hepatic metabolism disorder in zebrafish.
Different companies optimized their instruments with different reference dyes rox, fluorescein, etc. It is a compact, easy to use and flexible platform. Eppendorf mastercycler pro thermal cycler with controller. Mastercycler ep realplex from eppendorf biocompare product.
For completion, the following features concerning operation of the mastercycler ep realplex are not accessible to restricted users. I have spent the last few months researching real time pcr instruments, i i have been most impresses with the cepheid smart cycler ii. Pccontrolled versions of our range of epmotion automated pipetting systems, mastercycler ep realplex realtime pcr cycler our range of specialist micromanipulation equipment. This hard shell prevents the distortion and shrinkage that may occur when regular singlecomponent polypropylene pcr plates are exposed to the high temperatures of thermal cycling or heat sealing. Bt proteins cry1ah and cry2ab do not affect cotton aphid. Provide simple, flexible programming with choice of gradient or nongradient blocks. Topics covered include instrumentation, fluorescent chemistries, quantification strategies, data analysis software, environmental microbiology, water microbiology, food microbiology, gene expression. The eppendorf mastercycler pro thermal cycler brings you a flexible powerhouse with consistent heating and cooling. Mastercycler ep realplex software setup for the quantifast probe. The mastercycler nexus x2 is eppendorfs solution for busy pcr labs. Relative changes in gene expression were analyzed via 2 c t livak and schmittgen, 2001 by comparing transgenic versus control cells, using mastercycler ep realplex 2 software version 2. Eppendorf enewsletter subscribe to our email newsletter and you wont miss a thing.
Find eppendorf mastercycler ep realplex forums, questions, troubleshooting, discussions at labwrench productivity, performance, and flexibility small, fast and flexiblethe mastercycler ep realplex realtime pcr system meets. This eppendorf cyclemanager pro software allows control of up to 30 mastercycler pro with one computer. Most differences are obviously in the application software, especially in the way of data. This thermal cycler has standard slope technology allowing users to program their desired gradient across 12 rows with a spread of 124c, with a heating rate of 6cs and cooling rate of 4. Statistical significance between groups was determined by use of the students ttest. The assay was repeated at least 3 times with each template and the negative control. Comprehensive yet intuitive software paired with recent advances in realtime pcr technology make the mastercycler ep realplex a critical contribution to modern science and research. However, it can be difficult when you want to do something that you. Skirted 96well pcr plates life science research biorad. Realtime pcr was performed on mastercycler ep realplex eppendorf, germany by using the following thermal cycles. We enable science by offering product choice, services, process excellence and our people make it happen. Realmaster brings real sensitivity and reproduciblity in. A minimal number of moving parts in the optical detection module minimises the vulnerability of the overall system and promotes consistently high sensitivity in your realtime pcr experiments. It has the broadest detection, excitation range, is fully licenses and has 16 independantly progammable channels.